Genome-wide gene expression analysis in black South African women who develop gestational diabetes mellitus
To understand the impact of gestational diabetes on the foetus by investigating gene expression and DNA methylation to look for epigenetic perturbations that may lead to an unfavourable outcome in the offspring.
To assess differential genome-wide gene expression in:
1. Blood samples from women with GDM and controls.
2. Placental tissue biopsy samples from female/male neonates born to women with GDM (adverse in-utero environment) and those born to controls (healthy in-utero environment; absence of GDM).
To examine DNA methylation patterns in the subset of selected genes that show a significantly altered differential expression (≥2 fold).